Specialists
Years of experience in genetics, laboratory diagnostics and bioinformatics
for targeted therapy, immunotherapy and when hereditary cancer syndromes are suspected*
Request a callFor the selection of targeted cancer therapy
Molecular genetic methods are primarily used in oncology for diagnosis clarification, disease prognosis, and for selection of the most effective therapy (including targeted cancer therapy)
is the most advanced and promising method that allows performing whole genome, whole exome, and targeted sequencing. Sequencing multiple DNA fragments occurs in parallel, providing high information content at a relatively high speed. NGS can simultaneously assess the presence of various genetic abnormalities in DNA and RNA molecules, such as hotspot mutations (mutations with increased frequency in the population), single nucleotide variants (SNV), copy number variations (CNV), large insertions, deletions, and gene fusions.
Oncomine Focus Assay is an excellent example of an NGS assay used in cancer laboratory diagnostics.
Oncomine Focus Assay is a targeted next-generation sequencing (NGS), a multi-biomarker assay based on the Ion Torrent platform that detects variants across 52 cancer-relevant genes.
Other molecular genetic methods used in oncology
FISH (fluorescent in situ hybridization) - allows you to determine the exact location of the nucleotide sequences on the DNA or RNA, visualizing them with complementary fluorescent DNA probes. FISH detects chromosomal rearrangements (deletions, duplications, translocations, inversions). In oncology, it is most applicable to clarify the diagnosis and to determine tumor status when selecting therapy.
Detects chromosomal mutations: deletions, duplications, translocations, inversions
arrayCGH (comparative genomic hybridization on microarrays) is a method that detects unbalanced chromosomal rearrangements (deletions and duplications) of the entire genome. During the analysis, test and control DNA are labeled with different fluorescent dyes, mixed and hybridized on microarrays with DNA probes, quantitatively comparing the intensity of fluorescence.
PCR (polymerase chain reaction) is a simple and effective laboratory diagnostic method that allows multiple copies of specific DNA or RNA fragments to be multiplied for qualitative or quantitative assessment of the fragment sought. In oncology, it can be used to detect single-nucleotide genetic variants (allele-specific PCR) or microRNA. More often, however, PCR is part of more sophisticated diagnostic methods, such as sequencing. In addition, PCR is widely used to detect the presence of potentially carcinogenic pathogens (HPV types 16 and 18, EBV infections, HBV and HCV, retroviruses, etc.).
Detects the presence of potentially carcinogenic pathogens HPV types 16 and 18, EBV infection, HBV and HCV, retroviruses, etc.
NGS | FISH | aCGH | PCR | |
---|---|---|---|---|
Diagnosis of hereditary tumor syndromes | Yes | No | Yes | Yes |
Tumor types | Solid | Solid and hematologic | Solid and hematologic | Solid and hematologic |
Biomaterial used | Tissue | Blood, tissue, urine cell sediment | Tissue, blood | Any kind |
Limitations |
Not suitable for hematologic tumor types analysis |
Number of mutations examined in a single approach is limited Capable of detecting only large structural variations in the genome |
High quality requirements for DNA samples provided Tumor cell content must be greater than 50% |
A limited number of specific mutations in a particular gene are examined in a single assay |
Years of experience in genetics, laboratory diagnostics and bioinformatics
All data is strictly confidential and cannot be passed on to third parties
Results ready in a short time
Extensive control at each stage of testing
Free delivery of biomaterial across Russia
Email info@f-genetics.com for information